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Organic manufacturing process to extract the lycopene from ripe tomatoes at supercritical conditions of CO2. Organic mechanicl production system without chemical solvents and toxicity solutions to produce dietary supplements and others.

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Organic manufacturing process production of lycopene without solvents and any toxicity from ripe tomatos at supercritical conditions of CO2 at controlled environmental conditions producing "Organic Dietary Supplements" according to health standards, organic lycopene, the perfect organic antioxidant.

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Organic Lycopene Made in Italy
Tel: ++39 (333) 6371- 644

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MANUFACTURING PROCESS - ORGANIC LYCOPENE PRODUCTION TECHNOLOGY BY PIERRE SRL The most important manufacturing process of the dietary supplement system is the production of the "Organic Lycopene" because it's the key ingredient of each dietary, health, heart, food supplement produced by Pierre. Organic lycopene, the perfect and most powerful natural antioxidant extracted in supercritical conditions of CO2, is a unique innovative new product according to the production system and new technology used, our organic lycopene has a strong antioxidant power, to contrast the destructive action of free radicals in the human body, preventing human diseases. FOLLOWING BRIEF INTRODUCTION OF OUR MANUFACTURING PROCESS

ORGANIC PRODUCTION PROCESS The organic lycopene, produced by Pierre in our Italian facilities, is a high quality product obtained as result of an innovative and patented manufacturing process as procedure it has been patented by our company (World Patent number 0001354930), the lycopene production process is completelly solvent free and it has been developed and produced in an friendly environment.

SUPERCRITICAL CONDITIONS OF CO2 A supercritical fluid is obtained by submitting a gas to temperatures and pressures above its critical point. It can diffuse through solids like a gas but has the solvent properties of a liquid. Supercritical fluids are suitable as a substitute for organic solvents. Carbon dioxide (CO2 ) critical point is: 31°C and 73 bar

MAIN ADVANTAGES OF OUR ORGANIC MANUFACTURING PROCESS
The advantages of supercritical fluid extraction (compared with solvent extraction) are that it is relatively rapid because of the low viscosities and high diffusivities associated with supercritical fluids. The extraction can be selective to some extent by controlling the density of the medium and the extracted material is easily recovered by simply depressurizing, allowing the supercritical fluid to return to gas phase and evaporate leaving no or little solvent residues. Carbon dioxide (CO2) is the most common supercritical solvent. It is used on a large scale for the decaffeination of green coffee beans, the extraction of hops for beer production, and the production of essential oils and pharmaceutical products from plants. Moreover, the plants for supercritical fluid extraction are environmentally-friendly and have low risks for the operators which are not exposed to chemical solvents.

OUR ORGANIC LYCOPENE DOES NOT CONTAIN ANY TOXIC SUBSTANCE

ORGANIC LYCOPENE TOXICITY FREE Our Organic Lycopene doesn't contain any toxic substance before, during and after manufacturing process, our organic lycopene is obtained from organic red-ripe tomatoes certified ICEA. Tomatoes are pesticides-free, dioxins-free, heavy metals-free, OGM-free.
Organic lycopene is obtained at moderate temperature (70°C) with a process based on an exclusively physic principle (extraction with supercritical carbon dioxide)
Because this procedure does not involve the use of chemical solvents, the finished product is:
     - solvent-free
     - toxic reaction by-products-free
     - impurity-free
The organic lycopene, made in Italy by Pierre srl, is the unique lycopene 100% natural without any form of toxicity.

LYCOPENE MOLECULAR STRUCTURE - PHYSICAL STATE - LYCOPENE BIOAVAILABILITY IS HIGHLY INFLUENCED BY THE CRYSTALLINE STATE

ORGANIC LYCOPENE IS PRESENT IN EXTRACT PROCESS AS VISCOSE SOLUTION in lipids which are co-extracted with it.
The lycopene absorption and passage into the systemic circulation depends on crystal stability, organic lycopene being already in solution is promptly absorbed and available.
Lycopene bioavailability depends on the dimension of crystals. Recent studies demonstrated that smaller crystals are more easily dissolved in the human body and hence absorbed more rapidly than larger ones (500 nm crystals are 30% more absorbed than 5 mm ones).
ORGANIC LYCOPENE REPRESENTS THE BEST FORM FOR ITS ASSIMILATION because is fundamentally not present in a crystalline state.
Synthetic lycopene has a purity of 90-95%, it is in the form of large and regular crystals obtained after crystallization from a solution in chemical solvents and with low impurities content.
Natural lycopene has a purity of approximately 50-60%, it is in the form of small and not very regular crystals obtained after crystallization from a solution in chemical solvents and with higher impurities content.
LYCOPENE MOLECULAR STRUCTURE: ISOMETRIC CONFIGURATION
In nature, lycopene trans-form is more abundant (>90%) than cis-form, but in human tissues this ratio is the opposite (cis-isomer is > 60%). The cis-form is, in fact, more bioavailable due to a reduced ability to form large crystals with respect to the trans-form. The isomerization from trans-cis could take place during the extraction process or during digestive processes. The two isomeric forms have the same biological activity, but cis-form is more absorbed in human tissues than the trans -one.
A higher cis/trans ratio is strictly connected to a higher bioavailability.
Organic lycopene has a cis/trans ratio 10-20-fold higher than the ones of synthetic and natural lycopene.
PHYSICAL AND CHEMICAL PROPERTIES OF THE LYCOPENE MIXTURE: LIPID CONTENT
Carotenoids are absorbed by the human digestive system with the same emulsification mechanism that allows lipid assimilation. Their absorption depends also on their prompt release from food. The presence of oils or fats accelerates this process promoting the solubilisation of the carotenoid crystals enclosed in foods. Since lipids improves carotenoid absorption at the intestinal level, lycopene containing nutritional supplements include high percentages of vegetable oils. Moreover the absence

SEE FOLLOWING CHART WITH THE UNITED STATES FDA COMPARISON BETWEEN SYNTETIC, NATURAL AND ORGANIC LYCOPENE:

AT PIERRE WE PRODUCE, IN ITALY, DIETARY SUPPLEMENTS USING OUR OWN ORGANIC LYCOPENE

ORGANIC LYCOPENE IMPROVED ANTIOXIDANT AND ANTICANCER ACTIVITY

Certificato ICEA n. IT BIO ICA PL0279 del "Licopene da agricoltura biologica"

Web site of the "Ministero della Ricerca Italiana": www.ricercaitaliana.it → innovazione ed imprese → alcuni esempi → produzione biologica di licopene naturale → Licopene biologico → Pierre

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Patent WO/2008/015490 Title "Food supplement based on Biological Lycopene and process to obtain Biological Licopene"

Boileau AC, Merchen NR, Wasson K, Atkinson CA,.. -University of Illinois, Urbana-Champaign- USA. Cis-lycopene is more bioavailable than Trans-lycopene in vitro and in vivo in lymph-cannulated ferrets J. Nutr ; 1999, Jun.12

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Dr.A.Venket Rao. Tomatoes , Lycopene & Human Health - Preventing Chronic Diseases . Edited by Caledonian Science Press 2006

Ahuja KD, Pittaway JK, Ball MJ. School of Human Life Sciences, University of Tasmania, Launceston, Australia. Effects of olive oil and tomato lycopene combination on serum lycopene, lipid profile, and lipid oxidation . Nutrition. 2006 Jan 12

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Michael McClain R, Bausch J. - McClain Associates, Randolph, NJ, USA - Summary of safety studies conducted with synthetic lycopene . Regul Toxicol Pharmacol. 2003 Apr

Jhonson FJ, Qin J, Krinsky Ni, Russel RM - Ingestion by men of a combined dose of β-carotene and lycopene does not affect the absorption of β-carotene but improves that of lycopene . J Nutr.1997

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Agarwal S, Rao AV - Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Ont. Tomato lycopene and its role in human health and chronic diseases . CMAJ 2000 Sep 19

Lowe GM, Booth LA, Young AJ ,Bilton RF, School of Biomolecular Sciences, Liverpool.Lycopene and β-carotene protect against oxidative damage in HT29 cells at low concentrations but rapidally lose his capacity at higher doses .- Free Radic. Re. 1999 Beb 30(2) 141-51

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Fuhrman B, Volkova N. Rosenblat M, Aviram M. - Institute for Research in the Medical Sciences, Haifa, Israel. Lycopene synergistically inhibits LDL oxidation in combination with Vitamin E, glabridin, rosmarinic acid , carnosic acid , or garlic -Antioxid Redox Signal. 2000 Fall

Leticia G. Rao, et .., University of Toronto. Endocrinology Round. Will tomatoes prevent osteoporosis? February 2005 Volume 5, Issue 2.

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Laurie Budgar "FDA approves Lycopene Claim". Functional Foods & Nutraceuticals , Jan 2006

Zhou J.-R., Gugger , E.T. & Erdman,J.W (1996). The crystalline form of carotenes and the food matrix in carrot root decrease the relative bioavailability of beta end alfa Carotene in the ferret model. J.Am. Coll. Nutr. 15 : 84-91

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Thomas W. ,M.Boileau , Amy C. Boileau and John W.Erdman Jr. Bioavailaibility of all-trans and cis-isomers of lycopene .Experimental Biology and Medicine (2002) 227 :914-919

Myriam Richelle, Karlheinz Bortlik, Stephanie Liardet, Corinne Hager, Pierre Lambelet, Markus Baur, Lee A. Applegate and Elizabeth A. Offord - Department of Nutrition, Nestlè Research Center, Lausanne, Switzerland. A Food-Based Formulation Provides Lycopene with the Same Bioavailability to Humans as That from Tomato Paste. J. Nutr. 132: 404-408, 2002

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Rao, A. V. & Argawal, S. (1998) Bioavailability and in vivo antioxidant properties of lycopene from tomato products and their possible role in the prevention of cancer. Nutr. Cancer 3: 199-203

ORGANIC DIETARY AND HEALTH SUPPLEMENTS MANUFACTURING COMPANY TO THE HEALTH CARE INDUSTRY

IMPORTANCE OF LYCOPENE
Recent studies and scientific publications have demonstrated that lycopene has a significant antioxidant activity, twice higher than b-carotene and 10 times higher than vitamin E.
Antioxidants act against the damage caused by free radicals , protecting the body and preventing several diseases. Lycopene seems to be important in the prevention of cardiovascular diseases (atherosclerosis, hypertension, infarction, etc.), m any types of cancers (pancreas, digestive system, breast, liver, prostate), neuro degenerative diseases (Alzheimer's, Parkinson's), osteoporosis , diabete mellitus , male infertility , skin diseases (due to UVA-UVB rays), senile macular degeneration. Lycopene is one of the most important antioxidant in human health and wellness.

Since the human body cannot synthesize lycopene , it must be introduced eating fruits and vegetables or dietary supplements.  Most of the commercially available supplements contain lycopene which has been chemically synthesized or extracted from red-ripe tomatoes by the use of chemical solvents. In both cases, the final product contains residuals of solvents, which are toxic for the body

ORGANIC DIETARY AND HEALTH SUPPLEMENTS MANUFACTURING COMPANY TO THE HEALTH CARE INDUSTRY

ORGANIC LYCOPENE - PRODUCED BY PIERRE GROUP PATENT No. WO/2008/015490

LYCOPENE SUPPLEMENTS FOR DISTRIBUTORS AND PRIVATE LABEL As manufacturing industry we offer high quality certified organic dietary supplements, food supplements and health care supplement products for pharmaceutical treatment using our patented organic Lycopene, we are developing new organic dietary supplements to support world distribution market, furthemore we customized our products for Private Label market APPLY NOW

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